On‐site genetic analysis for species identification using lab‐on‐a‐chip

نویسندگان

چکیده

This paper presents a microfluidic device capable of performing genetic analysis on dung samples to identify White Rhinoceros (Ceratotherium simum). The development device, which can be used in the field, offers portable and cost-effective solution for DNA species identification aid conservation efforts. Optimization extraction processes produced equivalent yields compared conventional kit-based methods within just 5 minutes. use color-changing loop-mediated isothermal amplification reaction simultaneous detection cytochrome B sequence C. simum enabled positive results obtained as little 30 Field testing was performed at Knowsley Safari demonstrate real-world applicability biological samples.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

analysis of ruin probability for insurance companies using markov chain

در این پایان نامه نشان داده ایم که چگونه می توان مدل ریسک بیمه ای اسپیرر اندرسون را به کمک زنجیره های مارکوف تعریف کرد. سپس به کمک روش های آنالیز ماتریسی احتمال برشکستگی ، میزان مازاد در هنگام برشکستگی و میزان کسری بودجه در زمان وقوع برشکستگی را محاسبه کرده ایم. هدف ما در این پایان نامه بسیار محاسباتی و کاربردی تر از روش های است که در گذشته برای محاسبه این احتمال ارائه شده است. در ابتدا ما نشا...

15 صفحه اول

Species identification through mitochondrial rRNA genetic analysis

Inter-species and intraspecific variations in mitochondrial DNA (mtDNA) were observed in a bioinformatics analysis of the mitochondrial genomic sequences of 11 animal species. Some highly conserved regions were identified in the mitochondrial 12S and 16S ribosomal RNA (rRNA) genes of these species. To test whether these sequences are universally conserved, primers were designed to target the co...

متن کامل

Analysis of the Genetic Diversity in Citrus (Citrus spp.) Species Using SSR Markers

Abstract Determination of genetic diversity level is important in clarifying genetic relationships, characterizing germplasm and the registration of new cultivars. In this study, genetic variation among 56 accessions (G1~G56) of Citrus including several undefined local or native genotypes and some known varieties in Jiroft, Kerman province, Iran was investigated using SSR markers. In total, 12 ...

متن کامل

automatic verification of authentication protocols using genetic programming

implicit and unobserved errors and vulnerabilities issues usually arise in cryptographic protocols and especially in authentication protocols. this may enable an attacker to make serious damages to the desired system, such as having the access to or changing secret documents, interfering in bank transactions, having access to users’ accounts, or may be having the control all over the syste...

15 صفحه اول

A Novel Methodology for Structural Matrix Identification using Wavelet Transform Optimized by Genetic Algorithm

With the development of the technology and increase of human dependency on structures, healthy structures play an important role in people lives and communications. Hence, structural health monitoring has been attracted strongly in recent decades. Improvement of measuring instruments made signal processing as a powerful tool in structural heath monitoring. Wavelet transform invention causes a g...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Ecology and Evolution

سال: 2021

ISSN: ['2045-7758']

DOI: https://doi.org/10.1002/ece3.7053